Simultaneous Determination of Asperosaponin Ⅵ and Tanshinone ⅡA in Yigong Granules by HPLC
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1.Guiyang Food and Drug Control and Testing Center， Guizhou Guiyang 550081， China； 2.Guizhou Medical Products Administration Inspection Center， Guizhou Guiyang 550081， China
Objective： To establish a high-performance liquid chromatography (HPLC) method for the simultaneous determination of asperosaponin Ⅵ and tanshinone ⅡA in Yigong Granules， in order to improve the quality control standard of this preparation. Methods： HPLC analysis was performed using an Agilent Eclipse Plus C18 column (4.6 mm×250 mm， 5 μm). The mobile phase consisted of 0.1% (v/v) phosphoric acid solution (A) and acetonitrile (B)， with gradient elution according to a specified program. The flow rate was set at 1.0 mL·min-1， and the column temperature was maintained at 30 ℃. Detection wavelengths were set at 210 nm for asperosaponin Ⅵ and 270 nm for tanshinone ⅡA， based on their ultraviolet absorption characteristics. The injection volume was 10 μL. Results： Asperosaponin Ⅵ showed a good linear relationship within the concentration range of 23.575 0-707.250 0 μg·mL-1， with a correlation coefficient (r2) of 1.000 0. The average recovery rate was 96.4%， and the relative standard deviation (RSD) was 1.8% (n=6). Tanshinone ⅡA also exhibited a good linear relationship within the concentration range of 1.483 5-44.505 0 μg·mL-1， with an r2 of 1.000 0. The average recovery rate was 94.8%， and the RSD was 1.5% (n=6). Conclusion： A simple， accurate， reproducible， and stable HPLC method was successfully developed for the simultaneous and rapid determination of asperosaponin Ⅵ and tanshinone ⅡA in Yigong Granules. This method is reliable and practical， making it suitable for multi-component quality evaluation of the preparation.
