北豆根总生物碱纯化工艺研究

北豆根总生物碱纯化工艺研究

杨静伟,杨晶凡,李二林

黑龙江省药品审评认证中心,河南中医学院,黑龙江省药品审评认证中心

收稿日期:2013-05-17 修回日期:2013-05-27 出版日期:2013-06-25 发布日期:2013-06-24

Study on Purification Technology of Total Alkaloids from Rhizoma Menispermi

杨静伟, and

Heilongjiang Center for Drug Evaluation and Certification,,Heilongjiang Center for Drug Evaluation and Certification

Received:2013-05-17 Revised:2013-05-27 Online:2013-06-25 Published:2013-06-24

摘要/Abstract

摘要： 目的：优化北豆根总生物碱纯化工艺条件。方法：采用紫外分光光度法测定北豆根总生物碱含量，优选大孔吸附树脂最佳纯化分离工艺。结果：AB-8型大孔吸附树脂柱对北豆根总生物碱最大吸附量为0.7 g/g(药材量∶树脂湿重)，平均洗脱率66.56%，平均总生物碱转移率为 63.49%，最终产品的生物碱纯度达到 70.79%。结论：AB-8型大孔吸附树脂可用于纯化北豆根总生物碱，该方法简便可行。

Abstract: Objective: To optimize the purification technology of total alkaloids in rhizoma menispermi. Methods: Using the content of total alkaloids as the index determined by ultraviolet spectrophotometric, and the optimum purification process was determined by resin.Results: Each gram of AB-8 resin could absorb 0.7 g/g of the total alkaloids，and the desorption ratio was 66.56%.The transfer rate of total alkaloids was 63.49%.The product purity of total alkaloids was 70.79%.Conclusion: The AB-8 resin can be used for purificating total alkaloids from rhizoma menispermi and the established procedure is simple and feasible.

杨静伟,杨晶凡,李二林. 北豆根总生物碱纯化工艺研究[J]. .

杨静伟, and. Study on Purification Technology of Total Alkaloids from Rhizoma Menispermi[J]. .