盐酸安妥沙星对金黄色葡萄球菌和大肠埃希菌持续效应研究

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盐酸安妥沙星对金黄色葡萄球菌和大肠埃希菌持续效应研究

王琪,马池,吕媛,杨金辉,郑波

北京大学第一临床医院临床药理研究所,北京大学第一临床医院临床药理研究所,北京大学第一临床医院临床药理研究所,北京大学第一临床医院临床药理研究所,北京大学第一临床医院临床药理研究所

收稿日期:2013-05-25 修回日期:2013-05-25 出版日期:2013-06-25 发布日期:2013-06-24
基金资助:
国家科技重大专项课题(重大新药创制) 2012zx09303005-003

The In-Vitro Sustained Effects of Antofloxacin Hydrochloride against Staphylococcus Aureus and Escherichia Coli

Institute of clinical phamacology，Peking University First Hospital, Beijing 100191 China,,,,

Received:2013-05-25 Revised:2013-05-25 Online:2013-06-25 Published:2013-06-24

摘要/Abstract

摘要： 目的：研究新型氟喹诺酮类药物盐酸安妥沙星(antofloxacin hydrochloride，ANTO)对金黄色葡萄球菌和大肠埃希菌的持续效应：抗生素后效应(PAEs)、抗生素后亚抑菌浓度效应(PASMEs)和抗生素亚抑菌浓度效应(SMEs)。方法：采用宏量肉汤稀释法测定最小抑菌浓度(minimal inhibitory concentrations，MIC)。35℃环境中细菌暴露于抗菌药物2 h后用稀释法去除抗菌药物，以活菌计数法计数作细菌生长曲线，计算PAEs、PASMEs 和 SMEs。结果：盐酸安妥沙星对金黄色葡萄球菌和大肠埃希菌均呈现明显的PAEs、PASMEs和SMEs，且PASMEs和SMEs均呈现明显的浓度依赖特点。结论：盐酸安妥沙星对金黄色葡萄球菌和大肠埃希菌均可产生明显的PAEs、PASMEs和SMEs，提示制定给药方案时可考虑适当延长给药时间间隔。

Abstract: Objective: To study the sustained effects of new fluoroquinolones antofloxacin hydrochloride against Staphylococcus aureus and Escherichia coli, which consist of post antibiotic effects(PAEs)、post antibiotic subminimum inhibitory concentration effects (PASMEs) and Sub-inhibitory Effects(SMEs)．Methods: Minimal inhibitory concentrations(MIC)were determined by broth macro-dilution method. The antimicrobial agents were eliminated by dilution method after the bacteria were exposed to antimicrobials for 2h. Growth curves were followed by viable counts, and then PAEs、PASMEs and SMEs were calculated. Results：Antofloxacin hydrochloride had significant PAEs、PASMEs and SMEs against Staphylococcus aureus and Escherichia coli, and PASMEs and SMEs were distinct concentration-dependant. Conclusion：As the PAEs、PASMEs and SMEs induced by antofloxacin hydrochloride were obvious. It suggests that when preparing a prescription，we could prolong the medicine-administered interval time properly.

王琪,马池,吕媛,杨金辉,郑波. 盐酸安妥沙星对金黄色葡萄球菌和大肠埃希菌持续效应研究[J]. .