HPLC法对八珍益母丸中芍药苷和阿魏酸的含量

• 评价技术与方法 • 下一篇

HPLC法对八珍益母丸中芍药苷和阿魏酸的含量

马向东

河南省濮阳市食品药品检验所

收稿日期:2014-03-14 修回日期:2014-07-14 出版日期:2014-08-25 发布日期:2014-08-25

Determinae the Content of Paeoniflorin and Ferulic Acid in Bazhen Yimu Pills by HPLC

Henan Puyang Institute for Food and Drug Control,Henan

Received:2014-03-14 Revised:2014-07-14 Online:2014-08-25 Published:2014-08-25

摘要/Abstract

摘要： 目的：建立八珍益母丸中芍药苷和阿魏酸含量测定的方法。方法：采用高效液相色谱法,色谱柱为Kromasil C₁₈柱 (250mm?4.6mm,5μm)；乙腈—0.1%磷酸(18：82)为流动相；流速为1.0m L.min_-1；柱温35℃；检测波长为 230。结果：芍药苷在0.06492～0.6492 μg (r=0.9992 n=6 ),平均回收率为99.52%(RSD 1.53%,n=6)；阿魏酸在0.02652.～0.2652μg范围内线性关系良好(r=0.9996 n=6),平均回收率为103.70%(RSD 0.99%,n=6)。结论：该方法操作简单,分离度高,回收率好,适用于八珍益母丸的质量控制。

Abstract: Objective: To establish an method for determination of in Bazhen Yimu Pills . Methods：Kromasil C₁₈column(250mm×4.6mm,5μm)was used,Acetonitrile－0.1% phosphate acid(18:82) was used as mobile phase ,the flow rate was 1.0 m L.min_-1 ,Column temperature was 35 ℃ and UV detection was set at 230nm。Results： The calibration curve was linear with range of 0.06492 ~ 0.6492μg(r=0.999 2,n=6 )and 0.02652～0.2652μg (r=0.999 6,n=6 ) and average recovery was 99.52％,RSD＝1.53% for paeoniflorin and ferulic acid；The average recoveries were99.52%(RSD 1.53%,n=6),103.70%(RSD 0.99%,n=6)。 Conclusion：The method is simple, practicallty and convenient, accurate with a good reproducibility, can be used for determination of paeoniflorin and ferulic acid in Bazhen Yimu Pills.

马向东. HPLC法对八珍益母丸中芍药苷和阿魏酸的含量[J]. .