赵晓頔,刘云飞,杨利红*(黑龙江省食品药品检验检测所,哈尔滨 150088)

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赵晓頔,刘云飞,杨利红_*(黑龙江省食品药品检验检测所,哈尔滨 150088)

赵晓頔,杨利红

黑龙江省食品药品检验检测所,黑龙江省食品药品检验检测所

收稿日期:2014-11-14 修回日期:2015-01-12 出版日期:2015-02-25 发布日期:2015-01-25

Determination of the Content of Oxymetazoline Hydrochloride Nasal Drops by HPLC

HEILONGJIANG PROVINCE FOOD AND DRUG INSPECTIONG,

Received:2014-11-14 Revised:2015-01-12 Online:2015-02-25 Published:2015-01-25

摘要/Abstract

摘要： 目的建立HPLC法测定盐酸羟甲唑啉滴鼻液的含量。方法采用Diamonsil ODS C₁₈柱(4.6 mm?250 mm,5 μm),流动相为甲醇-水-三乙胺-磷酸(500：450：5：1.5),流速为1.0 mL.min_-1,检测波长279 nm,柱温25 ?C,进样量10 μL。结果盐酸羟甲唑啉在0.0512 mg.mL_-1～1.023 mg.mL_-1(r = 1.000)范围内线性关系良好,平均回收率为99.8%,RSD为1.2%(n = 9)。结论本方法简便准确,重复性好,能有效地控制盐酸羟甲唑啉滴鼻液的质量。

Abstract: Objective To develop a HPLC method for the determination of Oxymetazoline Hydrochloride Nasal Drops. Methods A Diamonsil C18 column(4.6 mm×250 mm,5 μm)was used with a mobile phase consisting of methanol-water-triethylamine-phosphoric acid (500:450:5:1.5,v/v) at a flow rate of 1.0 mL.min-1. The detection wavelength was set at 279 nm, and the column temperature was 25 °C. Results The liner range of Oxymetazoline Hydrochloride was 0.0512 mg.mL-1-1.023 mg.mL-1. The average recovery of Oxymetazoline Hydrochloride was 99.8%(RSD = 1.2%,n = 9). Conclusions The method can be used to control the quality of Oxymetazoline Hydrochloride Nasal Drops which provides the advantage of quickness, simplicity and repeatability.

赵晓頔,杨利红. 赵晓頔,刘云飞,杨利红_*(黑龙江省食品药品检验检测所,哈尔滨 150088)[J]. .