大七厘散的定性定量质量标准研究

大七厘散的定性定量质量标准研究

陈惠玲,胡珊梅,陈洁,李玲玲,黄惠琼

厦门市药品检验所,厦门市药品检验所,厦门市药品检验所,厦门市药品检验所,厦门市药品检验所

收稿日期:2014-12-19 修回日期:2015-02-26 出版日期:2015-04-25 发布日期:2015-04-25

Quality Standards for Daqili Powder

李玲玲 and

Xiamen institute for drug control,Xiamen institute for drug control,Xiamen institute for drug control,Xiamen institute for drug control,Xiamen institute for drug control

Received:2014-12-19 Revised:2015-02-26 Online:2015-04-25 Published:2015-04-25

摘要/Abstract

摘要： 目的大七厘散质量标准研究。方法建立大黄、当归、冰片、三七及骨碎补的薄层鉴别项。采用HPLC法对血竭中的主成分血竭素进行了含量测定，供试品溶液以12%磷酸甲醇溶液为溶媒的制备，流动相：乙腈-0.05mol/L磷酸二氢钠溶液（50：50）；检测波长：440nm。结果血竭素在0.774μg/ml～77.415μg/ml范围内呈良好的线性关系，r=0.9999。加样回收率为100.2%，RSD=1.8%（n = 9）。结论本法分离效果好，准确可靠，适用于大七厘散的质量控制。

Abstract: OBJECTIVE:To establish quality control for Daqili Powder. METHODS: Rhei Radix et Rhizoma, Angelicea sinensis Radix, Borneolum Syntheticum, Notoginseng Radix et Rhizoma , Drynariae Rhizoma in Daqili Powder were identified by TLC, and draorhodin was determined by HPLC. Test solution was made with 12% phosphoric acid in methanol,Use a mixture of acetonitrile and 0.05mol/L sodium dihydrogen phosphate solution (50:50) as the mobile phase. As detector a spectrophotometer set at 440 nm .RESDULTS: The calibration curves were linear within the range of 0.774μg/ml～77.415μg/ml（r=0.9999）.The average recovery was 100.2% with RSD was 1.8%（n = 9）.The calibration curves were linear within the range of 0.0997μg～1.9937μg（r=1.0000）. CONCLUSION: The method is sensitive and accurate, and it is suitable for the quality control of Daqili Powder.

陈惠玲,胡珊梅,陈洁,李玲玲,黄惠琼. 大七厘散的定性定量质量标准研究[J]. .

李玲玲 and. Quality Standards for Daqili Powder[J]. .