被动皮肤过敏性评价中阳性对照的建立

被动皮肤过敏性评价中阳性对照的建立

孙鸽,李炜宾,岳娟,尹晶晶,安全,闻建华

中国辐射防护研究院药物安全性评价中心,中国辐射防护研究院药物安全性评价中心,中国辐射防护研究院药物安全性评价中心,中国辐射防护研究院药物安全性评价中心,中国辐射防护研究院药物安全性评价中心,中国辐射防护研究院药物安全评价中心

收稿日期:2015-06-01 修回日期:2015-06-09 出版日期:2015-06-25 发布日期:2015-06-25

Establishment of positive controlin passive cutaneous anaphylaxis evaluation

闻建华

Drug Safety Evaluation Center in China Institute for Radiation Protection,Shanxi TaiYuan,030006,Drug Safety Evaluation Center in China Institute for Radiation Protection,Shanxi TaiYuan,030006,Drug Safety Evaluation Center in China Institute for Radiation

Received:2015-06-01 Revised:2015-06-09 Online:2015-06-25 Published:2015-06-25

摘要/Abstract

摘要： 目的：建立被动皮肤过敏性试验中准确且具可重复的阳性对照。方法：30只SD大鼠随机分为1个阴性对照(氯化钠注射液弗氏不完全佐剂)和4个阳性处理组(阳性1组：卵白蛋白,阳性2组：卵白蛋白百白破疫苗,阳性3组：卵白蛋白弗氏不完全佐剂,阳性4组：卵白蛋白弗氏完全佐剂及弗氏不完全佐剂),分别致敏5次制备抗血清,通过皮内注射被动致敏、48 h后激发,以皮内蓝斑有无及直径判定结果。结果：阴性对照和阳性1组、2组均未见蓝斑,阳性3和4组出现蓝斑,其1:2和1:8注射点蓝斑直径均大于5 mm。结论：被动皮肤过敏性试验中,致敏时结合使用弗氏佐剂可使阳性率达到100%,以保证试验的准确性和可重复性。

Abstract: Objective: To establish an accurate and reliable method for positive control of passive cutaneous anaphylaxis test (PCA test). Method: 30 SD rats were randomly divided into One negative control (Sodium Chloride Injection plus Freund"s adjuvant incomplete) and four positive treatment groups (the positive 1 group: Ovalbumin, the positive 2 group: Ovalbumin plus diphtheria-pertussis-tetanus (DPT) vaccine, the positive 3 group: Ovalbumin plus Freund"s adjuvant incomplete, the positive 4 group: Ovalbumin plus Freund"s adjuvant complete and Freund’s adjuvant incomplete) were set in the experiment. Firstly, antiserum was prepared with five subcutaneous injections. Secondly, passive sensitization was conducted by intradermal injection of antiserum. After 48 hours, the immuno-stimulation was followed by intravenous

孙鸽,李炜宾,岳娟,尹晶晶,安全,闻建华. 被动皮肤过敏性评价中阳性对照的建立[J]. .

闻建华. Establishment of positive controlin passive cutaneous anaphylaxis evaluation[J]. .