阿胶对围绝经期大鼠卵巢颗粒细胞凋亡及Bcl-2和Bax表达的影响

阿胶对围绝经期大鼠卵巢颗粒细胞凋亡及Bcl-2和Bax表达的影响

汝文文,和娴娴,钤莉妍,孙阳恩,王东亮,韩丽

国家胶类中药工程技术研究中心;山东东阿阿胶股份有限公司,国家胶类中药工程技术研究中心,国家胶类中药工程技术研究中心,国家胶类中药工程技术研究中心,国家胶类中药工程技术研究中心,

收稿日期:2014-12-09 修回日期:2015-04-08 出版日期:2015-06-25 发布日期:2015-06-25

Effect of Colla Corii asini on Ovarian Granulosa Cells Apoptosis and Expression of Bcl-2 and Bax in Perimenopausal RatWen-Wen Ru_1,2, Xian-Xian He_1.2, Guo-Hua Fan_1.2,Li-Yan Qian_1.2,Yang-en Sun_1.2,Don

韩丽

Shandong Dongeejiao CoLtd,Shandong Liaocheng,,,,,国家胶类工程中心，山东东阿阿胶股份有限公司

Received:2014-12-09 Revised:2015-04-08 Online:2015-06-25 Published:2015-06-25

摘要/Abstract

摘要： 目的：探讨阿胶对围绝经期大鼠卵巢颗粒细胞凋亡及其相关基因Bcl-2和Bax表达的影响。方法：选用4月龄雌性大鼠为正常对照组，14月龄阴道细胞学表现为动情期延长的雌性大鼠为衰老大鼠模型。模型动物随机分别给阿胶或戊酸雌二醇片治疗。采用原位末端核苷酸标记法（TUNEL）检测卵巢细胞凋亡，免疫组化法检测细胞凋亡相关因子Bcl-2，Bax。结果：阿胶高、中、低剂量组卵巢细胞凋亡阳性表达与模型组比较有显著性差异，同时可使卵巢凋亡基因Blc-2表达增强，促凋亡基因Bax表达减弱，Bcl-2/Bax比例增加。结论：阿胶通过抑制Bax蛋白的表达，上调Bcl-2蛋白表达，抑制卵巢颗粒细胞凋亡，进而改善卵巢功能。

Abstract: Objective: To study the effects of Colla Corii asini on ovarian granulosa cells apoptosis and expression of Bcl-2 and Bax in perimenopausal rats. Methods: SD female rats of 4 months old were set to be the normal control group and SD female rats of 14 months old whose oestrus elongation in colpocytology appearance were set as the senile female rat model. These rats were divided into 5 groups randomly, as follows: High dose Colla Corii asini group; Middle dose Colla Corii asini group; Low dose Colla Corii asini group; estradiol valerate group; Model group. After 8 weeks in the intervention period, ovarian apoptosis and the expression of ovarian granulosa cell Bcl-2, Bax were measured by TUNEL and Immunohisto-chemistry methods. Results: High and middle dose Colla Corii asini groups could decrease apoptosis in ovarian granulose cells (P<0.05), and could improve the expression of Bcl-2 in the ovaries and weakened the expression of Bax, which then increase the ratio of Bcl-2/ Bax. Conclusion: Colla Corii asini could delay ovarian granulose cells apoptosis through inhibiting the expression of Bax, increasing the expression of Bcl-2 during perimenopausal period, which might be one reason of Colla Corii asini postponed the ovarian failure and cured perimenopausal syndrome.

汝文文,和娴娴,钤莉妍,孙阳恩,王东亮,韩丽. 阿胶对围绝经期大鼠卵巢颗粒细胞凋亡及Bcl-2和Bax表达的影响[J]. .

韩丽. Effect of Colla Corii asini on Ovarian Granulosa Cells Apoptosis and Expression of Bcl-2 and Bax in Perimenopausal RatWen-Wen Ru_1,2, Xian-Xian He_1.2, Guo-Hua Fan_1.2,Li-Yan Qian_1.2,Yang-en Sun_1.2,Don[J]. .

参考文献

[1]孙思邈.千金方[M]. 呼和浩特：内蒙古人民出版社,2006:27-81
[2]麦秀军.黄连阿胶汤治疗更年期失眠的临床体会[J].现代诊断与治疗,2012,23(11)：1872-1873.
[3]路英,王文耀.黄连阿胶汤治疗更年期失眠14例[J].中国医药导报,2012,18(3)：93.
[4]张兰.黄连阿胶汤治疗女性更年期口干症40例的临床研究[J].贵阳中医学院学报,2011,33(4)：91-93.
[5]郑舟霞.针刺结合黄连阿胶汤辅助治疗更年期失眠临床观察[J].新中医,2013,45(8)：153-154.
[6]司徒仪.中西医结合妇产科学[M].北京：科学出版社,2003.189.
[7] J L Tilly, K I Kowalski, A L Johnson, A J Hsueh. Involvement of apoptosis in ovarian follicular atresia and postovulatory regression [J].Endocrinology, 1991, 129 (5): 2799 - 2801
[8] Kugu K , Ratts V S , Piquette G N , Tilly K I,Tao X J,Martimbeau S,Aberdeen G W,Krajewski S,Reed J C,Pepe G J,Albrecht E D,Tilly J L. Analysis of apoptosis and expression of bcl - 2 gene family members in the human and baboon ovary [ J ] . Cell death and differentiation, 1998, 5 (1): 67 - 76
[9]Perez G I, Robles R, Knudson C M, et al. Prolongation of ovarian life span into advanced chronological age by BAX deficiency, Nat Genet, 1999, 21(2):200-203.
[10] Tilly J L, Tilly K I, Kenton M L, et al. Expression of members of the bcl-2 gene family in the immature rat ovary: equine chorionic gonadotropin-mediated inhibition of granulosa cell apoptosis is associated with decreased bax and constitutive bcl-2 and bcl-xlong messenger ribonucleic acid levels [J]. Endocrinology, 1995, 136(1): 232-241.
[11] Durlinger A L, Kramer P, Karels B, Grootegoed J A, Uilenbroek J T, Themmen A P. Apoptotic and proliferative changes during induced atresia of pre - ovulatory follicles in the rat [J]. Human reproduction (Oxford, England), 2000, 15 (12): 2504 - 2511
[12] 张妙,邱晓晓,朱长玲等.补肾益冲抗衰汤对卵巢储备功能下降大鼠卵巢颗粒细胞凋亡因子Bcl-2、Bax、Caspase-3的影响[J].浙江中医药大学学报,2014,38(1)：68-72.
[13] 叶娜,董晓英,李冬华.卵巢早衰的颗粒细胞凋亡机制研究进展[J].首都医科大学学报,2014,35(3)：379-383.