Abstract: Objective：Establish the fingerprint of psoralea corylifolia by high performance liquid chromatography，to study the influence of different 60Co-γ irradiation doses on the effective components. Methods：Carry out the method for high performance liquid chromatography. Use Shim-Pack VP-ODS（4.6mm×250mm，5μm）column as detached dowel and a mixture of acetonitrile and water as mobile phase for gradient elution, the detection wavelength was set at 246nm. Results：Fingerprint was established with 8 common peaks, the similarity was in the range of 0.999-1.000, and methodology reached the required standard. Conclusion：The effective ingredients of fructus psoralea had no obvious change before and after 60Co-γ irradiation with different doses.