HPLC法测定复方杜仲丸中盐酸水苏碱与黄芩苷的含量

HPLC法测定复方杜仲丸中盐酸水苏碱与黄芩苷的含量

赵磊

四平市食品药品检验所

收稿日期:2017-03-10 修回日期:2017-03-31 出版日期:2017-04-25 发布日期:2017-04-25
基金资助:
四平市科技发展计划项目——中成药质量标准控制研究（NO.2014042）

Determination of Stachydrine Hydrochloride and Baicalin in Fufang Duzhong Pills by HPLC

Siping Institute food and Drug Control

Received:2017-03-10 Revised:2017-03-31 Online:2017-04-25 Published:2017-04-25

摘要/Abstract

摘要： 目的：建立HPLC法测定复方杜仲丸中盐酸水苏碱与黄芩苷的含量。方法：采用高效液相色谱（HPLC）法，强阳离子交换(SCX)色谱柱测定盐酸水苏碱的含量，十八烷基硅烷键合硅胶测定黄芩苷的含量。结果：盐酸水苏碱在0.01012～1.012 mg?mL-1、黄芩苷在0.00501～0.501 mg?mL-1浓度与峰面积具有良好的线性关系（r值均为0.9998）；盐酸水苏碱与黄芩苷回收率均符合要求。结论：该方法可控制复方杜仲丸中益母草与黄芩的含量。

Abstract: Objective： Establishment of a HPLC method for the determination of the contents of hydrochloric acid and baicalin in fufang duzhong pill. Methods：HPLC was used to determine the content of Stachydrine Hydrochloride with (SCX) column；HPLC was used to determine the content of baicalin with ODS column. Results： The Stachydrine Hydrochloride linear ranges were 0.01012~1.012 mg?mL-1 and Baicalin in the range of 0.00501～0.501 mg?mL-1. The recovery results were according to the requirement（r=0.9998）. Conclusion：The HPLC method can be used for the quality control of fufang duzhong pill.

赵磊. HPLC法测定复方杜仲丸中盐酸水苏碱与黄芩苷的含量[J]. .