HPLC波长切换法测定伸筋丹胶囊中柚皮苷、 4-甲氧基水杨醛和山奈素的含量

HPLC波长切换法测定伸筋丹胶囊中柚皮苷、 4-甲氧基水杨醛和山奈素的含量

江庆洋

信阳市食品药品检验所

收稿日期:2016-12-02 修回日期:2017-02-10 出版日期:2017-04-25 发布日期:2017-04-25

Determination of Naringin 4-methoxy Salicylaldehyde kaempferide in Shenjin Dan Capsules by HPLC with UV Switch

Xinyang Institute of Food and Drug Control

Received:2016-12-02 Revised:2017-02-10 Online:2017-04-25 Published:2017-04-25

摘要/Abstract

摘要： 目的：建立高效液相色谱（HPLC）法同时测定伸筋丹胶囊中柚皮苷、4-甲氧基水杨醛和山奈素3个成分的含量。方法：采用BDS-HYPERSIL C18色谱柱（4.6 mm×250 mm，5 μm），以乙腈-1%冰乙酸为流动相，梯度洗脱，流速1 mL?min-1，检测波长283 nm(0～10 min，测定柚皮苷)、278 nm（10～16.5 min，测定4-甲氧基水杨醛）、360 nm（16.5～30 min，测定山奈素）。结果：各待测成分分离度良好，柚皮苷、4-甲氧基水杨醛、山奈素的进样量分别在7.93～158.6 ng、6.85～137 ng、3.2～64 ng范围内与峰面积呈良好的线性关系；加样回收率分别为101.2%（RSD=1.0%）、99.3%（RSD=0.8%）、98.1%（RSD=1.5%）。结论：HPLC法简单、快速、灵敏、准确，可用于伸筋丹胶囊中柚皮苷、4-甲氧基水杨醛和山奈素的含量测定。

Abstract: Objective： To develop an HPLC method for simultaneous determination of naringin 4-methoxy salicylaldehyde kaempferide in Shenjin dan capsules. Methods： The chromatographic separation was carried out on a BDS-HYPERSIL C18 column(4.6 mm×250 mm，5 μm) with a mobile phase consisting of acetonitrile-1% glacial acetic acid in a gradient mode at a flow rate of 1 mL?min-1. The UV detection wavelength was set at 283 nm for naringin in the first 10 min，278 nm for 4-methoxy salicylaldehyde during 10～16.5 min，360 nm for kaempferide during 16.5～30 min. Results： Excellent chromatographic separation was achieved and the ranges for linear correlation of naringin 4-methoxy salicylaldehyde kaempferide were 7.93～158.6 ng(r2=0.9987)， 6.85～137 ng(r2=0.9999) and 3.2～64 ng(r2=0.9990)，respectively. The average recoveries of the three components were 101.2%(RSD=1.0%)， 99.3%(RSD=0.8%) and 98.1%(RSD=1.5%). Conclusion： The established method is simple，rapid， sensitive and accurate， which can be applied to determine naringin 4-methoxy salicylaldehyde kaempferide in Shenjin dan capsules.

江庆洋. HPLC波长切换法测定伸筋丹胶囊中柚皮苷、 4-甲氧基水杨醛和山奈素的含量[J]. .