Abstract: Objective To investigate the effects of puvecionine hydrochloride and atropine on respiratory secretions and hemodynamics in patients with gynecologic laparoscopic surgery. Methods A total of 100 patients underwent gynecologic laparoscopic surgery were randomly divided into two groups: penehyclidine hydrochloride group and atropine group. Two groups of patients were intravenous injection of penehyclidine hydrochloride and atropine before anesthesia. HR, MAP, and SpO2 were measured before and after treatment (T1), 10 min (T2) after administration, 20 min after administration (T3), 30 min after administration (T4). The degree of dryness was assessed by visual analogue scale (VAS), and the condition of oral and respiratory secretions before and after the removal of tracheal tube was observed and measured (mL). Results There was no significant difference in the change of HR at each time point after the administration of the tropine group (P> 0.05). HR at each time after administration of atropine group was significantly higher than that before administration (P <0.01), especially at 10 min after administration. The VAS scores of the two groups were increased after 30 min and after extubation (P <0.01),and the amount of endotracheal and nasal secretions in the atropine group was significantly higher than that in the long tonic group. Conclusion Penehyclidine hydrochloride is a safe and effective method for the treatment of gynecologic laparoscopic patients, and it is more safe and effective than atropine.