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HPLC法测定西瓜霜润喉片中胭脂红的含量

赵磊   

  1. 四平市食品药品检验所
  • 收稿日期:2018-02-14 修回日期:2018-04-09 出版日期:2018-04-25 发布日期:2018-04-25

Determination of neucoccin inXi gua shuang run hou tablet by HPLC

ZHAO Lei   

  1. Siping Institute food and Drug Control
  • Received:2018-02-14 Revised:2018-04-09 Online:2018-04-25 Published:2018-04-25

摘要: 目的:建立高效液相色谱(HPLC)法测定西瓜霜润喉片中胭脂红的含量。方法:采用HPLC法测定胭脂红,色谱柱为Agilent 5 TC-C18(4.6 mm×250 mm,5 μm,588925-902);乙腈-0.05 mol?L-1乙酸铵溶液 (20∶80)为流动相;检测波长为508 nm;流速1.0 mL?min-1;检测器:紫外;柱温:20 ℃;进样量:10 μL。结果:胭脂红在6.44~644 ng的范围内,线性关系良好(r=1.0000);平均回收率为99.17%(RSD=0.9%)。结论:该方法简单、准确、重现性好,可用于控制西瓜霜润喉片中辅料胭脂红的含量。

Abstract: Objective: To establish High Performance Liquid Chromatography(HPLC)method for determination of neucoccin in Xiguashuang Runhou tablet. Methods: HPLC was used to determine the content of neucoccin with Agilent 5 TC-C18(4.6 mm×250 mm,5 μm,588925-902)column . Acetylonitrile-0.05 mol?L-1 ammonium acetate (20∶80) was a mobile phase.The detection wavelength was 508 nm.The flow rate was 1.0 mL?min-1, and the column temperature was 20 ℃. Results: The linear range of neucoccin was 6.44~644 ng.The linear equations of neucoccin was in good linear range (r=1). The average recovery of neucoccin was 99.17%(RSD=0.9%). Conclusion: The method is simple, accurate and reproducible,and it can be used to control the quality of Xiguashuang Runhou table.