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消栓通络片中丹酚酸B的鉴别研究

崔业波   

  1. 四平市食品药品检验所
  • 收稿日期:2018-03-03 修回日期:2018-05-25 出版日期:2018-06-25 发布日期:2018-06-25

Study on the Identification of Salvianolic Acid B in Xiaoshuan Tongluo Tablet

cuiyebo   

  1. Siping City Institute for Food and Drug Control,Jilin Siping 136000
  • Received:2018-03-03 Revised:2018-05-25 Online:2018-06-25 Published:2018-06-25

摘要: 目的:初步探索消栓通络片中丹酚酸B的鉴别方法。方法: 采用HPLC法,以WondaCract ODS-2 (4.6 mm×250 mm,5 μm)色谱柱、乙腈-0.05%磷酸溶液为流动相,进行梯度洗脱;检测波长为286 nm;柱温为30 ℃;流速为1.0 mL?min-1,进样量为10 μL。 结果:4批样品中仅有一批样品检出丹酚酸B的色谱峰,并且与丹酚酸B对照品的光谱图一致。结论:该方法简单易行,专属性高,为消栓通络片质量标准的完善提供实验依据。

Abstract: Abstract Object :To establish methods for identification of Salvianolic acid B in Xiaoshuan Tongluo Tablet. Method : HPLC condition was as follows: WondaCract Objective: To establish an RP-HPLC method for identification of Salvianolic acid B in Xiaoshuan Tongluo Tablet. Methods: High performance liquid chromatography(HPLC)condition was as follows: A WondaCract ODS-2 (4.6 mm×250 mm,5 μm) column was used. A mobile phase that consisted of acetonitrile and 0.05% phosphoric acid was programmed for gradient elution at a flow rate of 1.0 mL?min-1. The detection wavelength was set at 286 nm and the column temperature was 30 ℃. Results: The chromatographic peaks of salicylic acid B were detected in only one sample, and were consistent with the spectrogram of salicylic acid B. Conclusion: The simple method has high specificity. It can be used to improve the quality standard of Xiaoshuan Tongluo Tablet.