Abstract: Objective： A high performance liquid chromatography (HPLC) method was established to study the content and uniformity of domestic cloperastine hydrochloride tablets. Methods： A Inertsil ODS-3（4.6 mm×100 mm，3 μm）column was used with methanol-0.1 mol·L-1 potassium dihydrogen phosphate solution-perchloric acid（60∶40：0.16） as the mobile phase， the detection wavelength was 222 nm，the sample volume was 10 μL. Results： Cloperastine hydrochloride had a good linear relationship within the range of 20.02~200.2 μg·mL-1（r=0.9993）. The average recovery of two enterprise were 100.2%（RSD=1.0%，n=9）and 99.5%（RSD=0.8%，n=9）， respectively．The contents of 106 batches of domestic cloperastine hydrochloride tablets were in the range of 82.7%~97.5%， and the average content was 88.0%. The contents of 89.6% domestic cloperastine hydrochloride tablets exceeds the limit （90.0%~110.0%）specified in the standard. The value of A+2.2S of the 106 batches of domestic cloperastine hydrochloride tablets were in the range of 8.60~27.30， and the average value was 21.04，the value of A+2.2S of 96.2% samples greater than 15.0. Conclusion： The method is specific， and can accurately determine the content and content uniformity of cloperastine hydrochloride tablets. The content and content uniformity of domestic cloperastine hydrochloride tablets were poor.