蒲公英总黄酮抑制Aβ25-35诱导的海马神经元细胞凋亡的研究

蒲公英总黄酮抑制Aβ25-35诱导的海马神经元细胞凋亡的研究

黄佳佳,张冰,王爱凤

阜外华中心血管病医院,阜外华中心血管病医院,阜外华中心血管病医院

收稿日期:2018-06-11 修回日期:2018-07-09 出版日期:2018-08-25 发布日期:2018-08-25

Total Flavonoids from Dandelion Inhibit Aβ25-35-induced Apoptosis in Hippocampal Neurons

huangjiajia, and

fuwai central china cardiovascular hospital,fuwai central china cardiovascular hospital,

Received:2018-06-11 Revised:2018-07-09 Online:2018-08-25 Published:2018-08-25

摘要/Abstract

摘要： 目的：研究蒲公英总黄酮用于防治阿尔兹海默症（AD）的机制，探讨蒲公英总黄酮对Aβ25-35诱导海马神经元细胞凋亡的影响，并进一步探究其潜在机制。方法：对海马神经元胞进行原代培养，经20 μmol·L-1 Aβ25-35诱导，细胞逐渐出现凋亡，用MTT法结合流式细胞仪，观察不同浓度的蒲公英总黄酮的细胞活力和细胞凋亡情况。用硫代巴比妥酸法、黄嘌呤氧化酶法来检测各组细胞中丙二醛（MDA）含量以及超氧化物歧化酶（SOD）活性。Western blot检测各组细胞凋亡相关的基因Bcl-2、Bax和Caspase-3的蛋白表达。结果：20 μmol·L-1 Aβ25-35组细胞比对照组活力下降，凋亡增加，MDA 含量上升，SOD 活性降低。与20 μmol·L-1 Aβ25-35组相比，各浓度下的蒲公英总黄酮均能够提高细胞活力，升高细胞内SOD活性，抑制细胞凋亡，降低MDA含量，同时上调Bcl-2表达，降低Bax和活化状态下的Caspase-3蛋白的表达，并呈浓度依赖性相关。结论：蒲公英总黄酮能够减少Aβ25-35诱导神经元细胞的细胞凋亡，这可能与拮抗其氧化损伤有关。

Abstract: Objective： To study the mechanism of total flavonoids in dandelion for the prevention and treatment of Alzheimer′s disease (AD). The studyinvestigated the effects of flavonoids from flavonoids on the apoptosis of hippocampal neurons induced by Aβ25-35 and further explored the underlying mechanisms. Methods： The primary hippocampal neuron was cultured in vitro and induced to apoptosis by 20μmol·L-1 Aβ25-35. The MTT and flow cytometry were used to observe the effect of total flavonoids from dandelion with different concentrations on cell viability and apoptosis in neurons. The MDA content and SOD activity were detected respectively by the thiobarbituric method and xanthine oxidase method. Western blot was used to detect expressions of apoptosis-related protein Bcl-2， Bax and caspase-3. Results： Compared with control group， 20μmol·L-1 Aβ25-35 could reduce the cell vitality significantly， increased the apoptosis and MDA contents， meanwhile decreased the SOD activity. Compared with the Aβ25-35 group， the cell survival rates were increased， the apoptotic rates were decreased， MDA contents were decreased and SOD activity were increased after pre-treatment with different concentrations of total flavonoids from dandelion. Furthermore， the protein expression of Bcl-2 was increased， while the protein expression of Bax and activated Caspase-3 were decreased in a dose-dependent manner. Conclusion： Total flavonoids from dandelion had protective effect on neurons against apoptosis induced by Aβ25-35， which may be related with the inhibition on oxidative damage.

黄佳佳,张冰,王爱凤. 蒲公英总黄酮抑制Aβ25-35诱导的海马神经元细胞凋亡的研究[J]. .

huangjiajia, and. Total Flavonoids from Dandelion Inhibit Aβ25-35-induced Apoptosis in Hippocampal Neurons[J]. .

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