灯盏乙素对过氧化氢致心肌细胞损伤的作用与机制研究

灯盏乙素对过氧化氢致心肌细胞损伤的作用与机制研究

白雪蕾

南阳市第二人民医院

收稿日期:2018-08-01 修回日期:2018-10-31 出版日期:2018-10-25 发布日期:2018-10-25

Protective Effects of Scutellarin on Oxidative Stress Injury of H9C2 Cardiomyocytes Induced by H2O2

baixuelei

Nanyang Second General Hospital,

Received:2018-08-01 Revised:2018-10-31 Online:2018-10-25 Published:2018-10-25

摘要/Abstract

摘要： 目的：探讨灯盏乙素对过氧化氢（H2O2）损伤心肌细胞的保护作用。方法：将大鼠H9C2心肌细胞分为对照组、H2O2 (200 μmol·L-1)干预组以及灯盏乙素(100、200 μmol·L-1)+H2O2 (200 μmol·L-1)干预组，每组设6个复孔。各组经过药物干预6 h后，通过 MTT法测定细胞存活率，流式细胞仪测定细胞凋亡率；测定细胞培养液中乳酸脱氢酶（LDH）、磷酸激酶（CK）、谷草转氨酶（AST）活性和丙二醛（MDA）含量；测定细胞中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）活性。结果：与对照组比较，H2O2干预组H9C2心肌细胞存活率显著降低且凋亡率显著升高，培养液中 LDH、CK、AST 活性和 MDA 含量均显著升高，细胞中 SOD、CAT、GSH-Px 活性显著降低，差异均具有统计学意义（P＜0.05）。与 H2O2干预组比较，灯盏乙素（100、200 μmol·L-1）+H2O2（200 μmol·L-1）干预组培养液中H9C2心肌细胞存活率显著升高、凋亡率显著降低，培养液中LDH 、AST、CK活性和MDA 含量均显著降低，细胞中SOD、CAT、GSH-Px活性显著升高，差异均具有统计学意义（P＜0.05）。结论：灯盏乙素能够有效提高 H2O2诱导状态下 H9C2 心肌细胞存活率并降低凋亡率，改善细胞中抗氧化酶活性、降低细胞损伤，提示灯盏乙素对H2O2诱导 H9C2心肌细胞氧化应激损伤具有剂量相关性的保护作用。

Abstract: Objective： To investigate the protective effects of scutellarin on oxidative stress injury of H9C2 cardiomyocytes induced by H2O2. Methods：H9C2 cardiomyocytes were randomly divided into control group， H2O2 (200 μmol·L-1) group， scutellarin (100 μmol·L-1) +H2O2 (200 μmol·L-1) group， and scutellarin (200 μmol·L-1) +H2O2 (200 μmol·L-1) group， and each group set six hours. After H2O2 stimulation for 6 h， the survival rate and the apoptosis rate were detected by MTT method and flow cytometry assay， respectively. The activity of LDH， CK， & AST， and the content of MDA in culture medium were detected， and the activities of SOD， CAT， and GSH-Px in cardiomyocytes were also determined. Results： Compared with the control group， the survival rate H9C2 cardiomyocytes in H2O2 (200 μmol·L-1) group was significantly decreased， and the apoptosis rate was significantly increased (P<0.05). The activity of LDH， CK， & AST， and the content of MDA in culture medium were significantly increased (P<0.05)， the activity of SOD， CAT， and GSH-Px in cardiomyocytes were significantly decreased (P<0.05). Compared with the H2O2 group， the survival rate of scutellarin (100， 200 μmol·L-1) +H2O2 (200 μmol·L-1) groups was significantly increased， and the apoptosis rate was significantly decreased (P<0.05)， the activity of CK， AST， LDH and the content of MDA in culture medium were significantly decreased (P<0.05)， and the activity of SOD， CAT， and GSH-Px in cardiomyocytes were significantly increased (P<0.05). Conclusion： Scutellarin can effectively increase the survival rate of H9C2 cardiomyocytes treated with H2O2， and decrease the apoptosis rate， improve the activity of antioxidase， and depress the cell injury， which suggests that scutellarin has dose-dependent protective effects against the oxidative stress of H9C2 cardiomyocytes induced by H2O2.

白雪蕾. 灯盏乙素对过氧化氢致心肌细胞损伤的作用与机制研究[J]. .

baixuelei. Protective Effects of Scutellarin on Oxidative Stress Injury of H9C2 Cardiomyocytes Induced by H2O2[J]. .

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