Abstract: Objective：To establish and validate the method for simultaneously determining the content of artemether and lumefantrine in the artemether and lumefantrine dispersible tablets.Methods： Using a stainless steel column (15 cm×3.9 mm) packed withparticles of silica gel which has been modified with chemically bonded octadeylsilyl groups (5 μm). 70 volumes of ion pair reagent and 30 volumes of acetonitrile R was the mobile phase A， 30 volumes of ion pair reagent and 70 volumes of acetonitrile R was the mobile phase B， and gradient elution. Operate with a flow rate of 1.3 mL per minute. As a detector using an ultraviolet spectrophotometer was set at a wavelength of about 210 nm for the first 28 minutes and then switch to about 380 nm. Results：There was a good linear relationship for artemether or lumefantrine in the range of 0.051 0~0.611 9 mg·mL-1 and 0.299 2~2.392 7 mg·mL-1， respectively. The correlation coefficients were 0.999 9 and 0.999 5， respectively. The average spiked recoveries were 100.2% and 99.6%， respectively. Conclusion：The method can simultaneously determine the content of artemether and lumefantrine in the artemether and lumefantrine dispersible tablets， and which is simple， accurate， rapid， good in separation， high in stability and strong in specificity.