Abstract: Objective： A comprehensive analytical method using ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was developed for screening rapidly of 3 imidazoline compounds (naphazoline hydrochloride, oxymetazoline hydrochloride, xylometazoline hydrochloride) added in anti-rhinitis pharmaceutics. Methods: After nasal drops, nasenspray and nasal pastewere were extracted with solvents and filtered, the solutions would be separated on a Agilent ZORBAX Extend C18 column（2.1 mm×50 mm，1.8 μm）and screened analysis carried out by electrospray ionization quadrupole time-of-flight mass spectrometry (Q-TOF/MS) with in 5 min. Results: The limits of detection for 3 compounds were from10.0 ng·mL-1 to 26.3 ng·mL-1, the mean recoveries at three spiked levels was from 92.6% to 104.0%, and the relative standard deviations were from 1.68% to 4.65%. Conclusion: The method is rapid, simple and accurate, and provides a reliable and effective technique for high-throughput screening of anti-rhinitis preparations.