关键词: font-size:medium, ">延胡索饮片；柠檬黄；日落黄；金橙Ⅱ；金胺O；酸性黄36

Abstract: Objective： To establish a method for the determination of five yellow pigments (Lemon yellow， Sunset yellow， Orange Ⅱ， Auramine O， Acid yellow 36) added into corydalis rhizoma pieces by HPLC-MS/MS. Methods： The analysis was performed with Aglient triple quadrupole mass spectrometer.Electrospray ion(ESI)was adopted as the ion source with positive and negative ion scanning mode(MRM).The Aglient Eclipse Plus C18 (2.1 mm×100 mm， 2.2 μm) column was used with acetonitrile -0.02 mol·L-1 ammonium acetate solution as the mobile phase.The flow rate was 0.3 mL·min-1， the columm temperature was 40 ℃. Results： The result showed good linearity in the ranges of 0.40-20.00 μg·mL-1 for lemon yellow and sunset yellow， 0.05-2.50 μg·mL-1 for orange Ⅱ， auramine O and acid yellow 36. The limits of detection were 0.1 μg·mL-1 and 0.02 μg·mL-1.The average recovery of five yellow pigments was in the range of 89.6%-97.5%．None of the above five yellow pigments were detected in 10 batches of corydalis rhizoma pieces. Conclusion： The method is convenient， reliable and accurate， and can be used for the detection of yellow pigments in corydalis rhizoma pieces. It′s necessary for the quality control of corydalis rhizoma pieces.

Key words: font-size:medium, ">Corydalis rhizoma pieces； Lemon yellow； Sunset yellow； Orange Ⅱ； Auramine O； Acid yellow 36