关键词: font-size:medium, ">不确定度评价；核黄素；核黄素磷酸钠；PHLC法

Abstract: Objective： To evaluate the measurement uncertainty on determination of riboflavin in riboflavin sodium phosphate， and the factors that affect the uncertainty by high performance liquid chromatography( HPLC). Methods： A mathematical model for the determination was established，the factors affecting the uncertainty was found，and the related uncertainty components were evaluated. The synthetic uncertainty and the expanded uncertainty of the determination by HPLC were obtained after analyzing the relative standard uncertainty of each component. Results： The content of riboflavin in riboflavin sodium phosphate was 2.90 %，the synthesis uncertainty in the determination was 0.011 %，the measurement result was expressed as (2.90±0.011) %. Conclusion： The method of the uncertainty evaluation for the determination of riboflavin in riboflavin sodium phosphate by HPLC method is established. The main sources of uncertainty are analyzed. This research provides a reliable theoretical basis for effectively controlling the accuracy of the determination method of sodium ion content.

Key words: Uncertainty evaluation, Riboflavin, Riboflavin sodium phosphate, High performance liquid chromatography(HPLC)