HPLC-PDA法同时测定金嗓子喉片中没食子酸和绿原酸的含量

HPLC-PDA法同时测定金嗓子喉片中没食子酸和绿原酸的含量

刘静,董宇,母芹,王震红

辽宁省药品检验检测院,辽宁省药品检验检测院,沈阳药科大学,辽宁省药品检验检测院

收稿日期:2018-09-11 修回日期:2018-09-11 出版日期:2018-12-25 发布日期:2018-12-25

Simultaneous Determination of Gallic Acid and Chlorogenic Acid in Golden Throat Lozenge by HPLC-PDA

LIU Jing,, and

Liaoning Institute for Drug Control,Liaoning Institute for Drug Control,Shenyang Pharmaceutical University,Liaoning Institute for Drug Control

Received:2018-09-11 Revised:2018-09-11 Online:2018-12-25 Published:2018-12-25

摘要/Abstract

摘要： 目的：建立了一种同时测定金嗓子喉片中没食子酸和绿原酸含量的二极管阵列检测器色谱（HPLC-PDA）方法。方法：采用资生堂C18色谱柱(4.6 mm×250 mm，5 μm)，以乙腈-0.1%磷酸水为流动相进行梯度洗脱，流速为1.0 mL·min-1，柱温为25 ℃，检测波长设为270 nm（没食子酸）和330 nm（绿原酸）。结果：没食子酸与绿原酸浓度分别在10.02~80.16μg·mL-1（r=0.999 8）和5.973~47.78 μg·mL-1（r=0.999 7）范围内与峰面积呈良好的线性关系，平均回收率分别为100.3%（RSD=0.59%）和99.6%（RSD=0.85%），待测液在室温的条件下，60 h之内保持稳定。结论：本方法专属性强，重复性好，结果准确，可用于金嗓子喉片中没食子酸、绿原酸的含量测定。

Abstract: Objective：High performance liquid chromatography-photodiode array(HPLC-PDA) method was developed for the determination of gallic acid and chlorogenic acid in Golden Throat Lozenge.Methods： The separation of gallic acid and chlorogenic acid was performed on a SHISEIDO HPLC PACKED C18 column（4.6 mm×250 mm， 5 μm）with the mobile phase consisting of acetonitrile-0.1%phosphoric acid in a gradient elution mode at a flow rate of 1.0 mL·min-1. Ultraviolet absorption detector was set at 270 nm for gallic acid and 330 nm for chlorogenic acid. The column temperature was set at 25 ℃.Results： Good linearity was obtained in the ranges of 10.02-80.16 μg·mL-1（r=0.999 8）for gallic acid and 5.973-47.78μg·mL-1（r=0.999 7）for chlorogenic acid， respectively. The spiked recoveries were 100.3% and 99.6% with the relative standard derivations (RSDs) of 0.59% and 0.85% for gallic acid and chlorogenic acid， respectively. The limits of quantification (LOQs)(S/N=10) were 0.1 μg·mL-1 and 0.06 μg·mL-1， and the limits of detection (LODs) (S/N=3) were 0.06 μg·mL-1 and 0.01 μg·mL-1 for gallic acid and chlorogenic acid， respectively. Conclusion：The results demonstrated that the proposal method is simple， accurate， reproducible and suitable for quality control of Golden Throat Lozenge.

刘静,董宇,母芹,王震红. HPLC-PDA法同时测定金嗓子喉片中没食子酸和绿原酸的含量[J]. .

LIU Jing,, and. Simultaneous Determination of Gallic Acid and Chlorogenic Acid in Golden Throat Lozenge by HPLC-PDA[J]. .

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