Abstract: Objective：To investigate the cases of drug-induced liver injury（DILI） in a hospital. Methods： A retrospective analysis of the clinical diagnosed of 152 patients with drug-induced liver injury was performed， including medication status， gender， age distribution， clinical manifestations and classification， related biochemical indicators and pathological characteristics. Results： The patients who diagnosed with drug-induced liver injury with high frequency of clinical symptoms were weak， poor， jaundice. ALT number of hepatocyte DILI and ALP number of cholestatic DILI were the highest (P<0.05). The most common chemical medicine in drug-induced liver injury were antineoplastics， tuberculostatics， NSAIDs， antibiotics， etc. The traditional Chinese medicine that caused drug-induced liver injury were polygonum multiflorum， pseudo-ginseng and Tripterygium wilfordii. Conclusions： Pharmacists should strengthen the screening and judgment of drug-induced liver injury cases， and train clinical medical personnel in the diagnosis and differential diagnosis of such adverse drug reactions， thereby reducing or avoiding the occurrence of drug-induced liver injury.