HPLC法测定桑叶枇杷阿胶膏中芦丁的含量

HPLC法测定桑叶枇杷阿胶膏中芦丁的含量

朱海芳,海娟,王春艳,李民,郑海荣,段桂峰

山东东阿阿胶股份有限公司国家胶类中药工程技术研究中心,山东东阿阿胶股份有限公司国家胶类中药工程技术研究中心,山东东阿阿胶股份有限公司国家胶类中药工程技术研究中心,山东东阿阿胶股份有限公司国家胶类中药工程技术研究中心,山东东阿阿胶股份有限公司国家胶类中药工程技术研究中心,山东东阿阿胶股份有限公司国家胶类中药工程技术研究中心

收稿日期:2014-06-06 修回日期:2014-08-04 出版日期:2014-08-25 发布日期:2014-08-25

Determination of Content of Rutin in ShangyePipaEjiao paste by HPLC

National Engineering Technology Research Center of Glue of Traditional Medicine，Shan Dong Dong-E E-Jiao Co.,Ltd,,,,,

Received:2014-06-06 Revised:2014-08-04 Online:2014-08-25 Published:2014-08-25

摘要/Abstract

摘要： 目的: 建立胶类复方制剂桑叶枇杷阿胶膏中芦丁的HPLC含量测定方法。方法:色谱柱：Waters SunFireC₁₈柱(250mm?4.6mm,5μm)；流动相：甲醇-0.5%磷酸溶液(梯度洗脱)；流速为1.0mL/min；检测波长为358nm。结果：芦丁在0.1025～2.0500μg范围内与其峰面积呈良好线性关系(r＝0.9999),平均加样回收率为98.63％,RSD为2.27%。结论：该测定方法简单易行,结果准确,重复性好,可用于测定桑叶枇杷阿胶膏中芦丁含量。

Abstract: objective: To establish the HPLC method for determination the content of rutin in ShangyePipaEjiao paste. Method: Waters SunFireC₁₈ column (250mm× 4.6mm,5μm) column was used with the mobile phase composed of methanol- phosphoric acid solution(0.5%)( gradientSelute) at the flow rate of 1.0mL/min. The detection wavelength was 358 nm. Result: Rutin showed a good linear relationship to the peak aera at a concentration rang of 0.1025～2.0500μg(r=0.9999). The average recovery was 98.63%, with RSD of 2.27%. Conclusion: The method is accurate, reliable, sensitive, simple and suitable for the determination of content of rutin in ShangyePipaEjiao paste.

朱海芳,海娟,王春艳,李民,郑海荣,段桂峰. HPLC法测定桑叶枇杷阿胶膏中芦丁的含量[J]. .