关键词: 超高效液相色谱-质谱联用, 多反应监测, 甲氨蝶呤, 银屑病

Abstract: Objective：To establish a method for the quantitative determination of Methotrexate in the Treatment of psoriasis Health Products by UPLC-MS/MS. Methods： Chromatography was carried on a Phenomenex Kinetex C18（2.1 mm×100 mm，1.7 μm） with the column temperature at 30 ℃， using 0.1% formic acid water solution-methon gradient elution as mobile phase at a flow rate of 0.3 mL·min-1. Determination was performed by multiple reaction monitoring mode using electrospray ionization in positive ion mode. Results：Methotrexate had good linear relationships in 0.5~500 μg·L-1(r=0.999 6)， average recoveries were 95.67%~98.59%. The limits of quantitation and detection were 0.5 μg·L-1 and 0.2 μg·L-1， respectively. Conclusion：The method is rapid， sensitive and accurate， and could be an appropriate technique to detect the illegal addition of health products.

Key words: UPLC-MS/MS, Multiple reaction monitoring, Methotrexate, Psoriasis