关键词: font-size:medium, ">固相萃取；高效液相色谱法；滑膜炎片/颗粒；防己诺林碱；粉防己碱；SPSS

Abstract: Objective： To establish a method for thedeterminationof tetrandrine and fangchinoline in Huamoyantablets/granules.Methods： Toestablishthe conditions ofsolid-phase extraction. Finally， a 0.1 mol·L-1 hydrochloric acid solution and methanol were selected as the cleaning solution， and a newly prepared acetonitrile-strongammonia solution (95∶5) was used as the eluent， which was basically eliminatedthe influence of interference components on the measurement， the background absorption of the chromatogram is small， and the baseline is stable； a SePax HP-C18 (4.6×150 mm， 5 μm) column is used； mobile phase： methanol-0.1% triethylamine (78∶22)； flow rate： 1.0 mL·min-1； detection wavelength is 280 nm； column temperature： 35 ℃. Results： The concentration of tetrandrine in the range of 2.50～24.99μg·mL-1 (r=0.999 9)， the concentration of fangchinoline in the range of 5.10～50.99 μg·mL-1 (r=0.999 9) showed a good linear relationship with the peak area； the average ratesofrecovery：99.7% of tetrandrine(n=6) and100.3% of fangchinoline(n=6). Conclusion： This method is simple， accurate and suitable for the quality control of Huamoyan tablets/ granules.

Key words: font-size:medium, ">Solid-phase extraction； HPLC； Huamoyan tablets/granules； Tetrandrine； Fangchinoline； SPSS