关键词: font-size:medium, ">壮药制剂；痛风立安颗粒；急性毒性试验；最大耐受量

Abstract: Objective： To observe the acute toxicity of the Tong feng li′an Granule and to provide experimental data for safety use in clinical. Methods： According to the classic mouse acute toxicity test method， Tongfeng Li′an Granules are administered by oral gavage at 40 mL·kg-1， and the poisoning performance and characteristics of each group of animals are carefully observed and recorded within 0～4 h after administration. The appearance and recovery time and death， etc.， were observed continuously for 14 days. The animals were weighed before and on the 4th， 7th， 10th and 14th days after the administration on the day of administration， and the changes in the body weight of the animals were recorded. Results：The animal toxicity of each dose group of Tongfeng Li′an Granules was positively correlated with the dose administered， mainly manifested as decreased exercise， diarrhea， proneness and death. Among them， the group of Tongfeng Li′an Granules Ⅰ， Ⅱ， Ⅲ and Ⅳ (103.6 g crude drug·kg-1， 120.4 g crude drug·kg-1， 142.8 g crude drug·kg-1， 168.0 g crude drug) 0/10， 0/10， 3/10 and 5/10 animals died respectively. There were no obvious abnormalities and deaths in the surviving animals of each dose group from the afternoon of the next day to the 14th day after the administration， and there was no significant effect on the body weight of the mice. There were no obvious abnormalities on the surfaces and sections of the organs of the dead animals and the planned anatomical animals. Conclusion：The main acute toxicity after animal administration is diarrhea， decreased exercise， proneness， and death， suggesting that the target organ may be the gastrointestinal tract. The maximum tolerated dose (MTD) is ≤120.4 g crude drug·kg-1， which is equivalent to Adult clinical dose 99 times (according to kilogram body weight) and 13 times (according to body surface area).

Key words: font-size:medium, ">Traditional medicine； Tongfeng Li′an Granules； Acute toxicity； Maximum tolerance (MTD)