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中国药物评价 ›› 2021, Vol. 38 ›› Issue (1): 59-61.

• 评价技术与方法 • 上一篇    下一篇

HPLC法同时测定喘嗽宁片中橙皮苷和黄芩苷的含量

  马晓静, 程焱, 赵艳*   

  1. 四平市食品药品检验所, 吉林 四平 136000
  • 收稿日期:2020-05-14 修回日期:2020-05-26 出版日期:2021-02-28 发布日期:2021-02-28

HPLC Determination Hesperidin and Baicalin in Chuansouning Tablets

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  1. Siping City Institute for Food and Drug Control, Jilin Siping 136000, China
  • Received:2020-05-14 Revised:2020-05-26 Online:2021-02-28 Published:2021-02-28
  • Contact: ma xiaojing E-mail:250225317@qq.com

摘要: 目的:建立HPLC法同时测定喘嗽宁片中橙皮苷和黄芩苷的含量。方法: 采用Hydrosphere C18色谱柱(4.6 mm×250 mm,5 μm),流动相为乙腈(A)-0.1%磷酸(B)线性梯度洗脱,检测波长280 nm,柱温30 ℃,流速1.0 mL·min-1,进样量为5 μL。结果:黄芩苷、橙皮苷分别在:117.12~702.72 ng、64.42~386.52 ng范围内均呈良好的线性关系;平均回收率(n=6)分别为101.12%、96.24%。结论:本方法可用于测定喘嗽宁片中橙皮苷和黄芩苷的含量,结果准确,重复性好。

关键词: font-size:medium, ">喘嗽宁片;橙皮苷;黄芩苷;高效液相色谱

Abstract: Objective: To establish an HPLC method to determine the content of hesperidin and baicalin in Chuansouning Tablets. Methods: The optium HPLC condition was as follows: Hydrosphere C18(4.6 mm×250 mm, 5 μm) column was used with a mobile phase of acetonitrile(A)-0.1% phosphoric acid(B). The flow rate was 1.0mL ·min-1. The detection wavelength was 280 nm.And the column temperature was 30 ℃. Resules: There were good linearity between 117.12-702.72 ng and 64.42-386.52 ng. The average recoveries were 101.12% and 96.24%. Conclusion: The method could detect hesperidin and baicalin in Chuansouning tablets . The HPLC measurement was reasonable,stable and accurate.
   

Key words: font-size:medium, ">Chuansouning tablets; Hesperidin; Baicalin; High performance liquid chromatography(HPLC)

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