关键词: font-size:medium, ">口服水解蛋白；质量评价；分子量；游离氨基酸；肽

Abstract: Objective： To evaluate the quality of oral protein hydrolysate， and improve the quality standard.Methods： 23 batches of oral protein hydrolysate were tested according to the legal standard.The molecular weight distribution of oral protein hydrolysate was determined by size exclusion chromatography. An ICP-AES method was established for the determination of calcium， zinc and iron .The content of amino acids in oral protein hydrolysate was determined by ion exchange chromatography with ninhydrin post-column derivatization.Results： The results showed the molecular weight of oral protein hydrolysate is about 240 Da. The calcium content of oral protein hydrolysate ranged in 3 293-5 841μg·g-1and the zinc content ranged in 32-74 μg·g-1 and the iron content ranged in 13-34 μg·g-1.It contained 18 kinds of amino acids， the total amount of free amino acids ranged in 40.3%-49.3%. The peptide content ranged in 15.4%-34.7%. Conclusion： The quality of oral protein hydrolysate is generally good. It is suggested that relevant enterprises pay attention to the quality of raw materials， and that microbial limit test and free amino acid content determination should be added to the quality standards， as well as the regulation of peptide limit， so as to ensure the stability of the production process and the standard can be controlled.
    

Key words: Oral protein hydrolysate, Quality evaluation, Molecular weight, Free amino acid, Peptide