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中国药物评价 ›› 2021, Vol. 38 ›› Issue (6): 513-517.

• 评价技术与方法 • 上一篇    下一篇

HPLC法测定羊毛甾醇含量研究

 钱绍安,梁云,臧广喜,陈钧,邓瑞云   

  1. 广州慧柏瑞生物医药科技有限公司,广东 广州 510700
  • 收稿日期:2021-01-05 修回日期:2021-05-06 出版日期:2021-12-28 发布日期:2021-12-28
  • 基金资助:
    广东省珠江人才计划领军人才项目(项目编号:2016LJ06Y375)

Determination of Lanosterol Content by HPLC Method

  1. Guangzhou Hui Bo Rui Biological Pharmaceutical Technology Co., Ltd, Guangdong Guangzhou 510700,China
  • Received:2021-01-05 Revised:2021-05-06 Online:2021-12-28 Published:2021-12-28

摘要: 目的:建立羊毛甾醇的含量测定方法。方法:实验采用InfinityLab Poroshell 120 Ec-C18(4.6×150 mm,5 μm)色谱柱;以甲醇为流动相,流速为1.0 mL·min-1;检测波长为210 nm;进样量为10 μL;柱温35 ℃。结果:羊毛甾醇峰与其他杂质峰可达到完全分离,检测限为0.008 mg·mL-1;浓度在0.048 49~0.113 1 mg·mL-1范围内与峰面积线性关系良好(相关系数为:1.000 0);重复性良好,RSD为1.2%;改用GL Sciences Intertsil ODS-SP C18(4.6×150 mm,5 μm)色谱柱测定对照品峰面积RSD为0.30%。结论:本研究建立的方法快速简便,灵敏度高,专属性强,重现性好,可用于羊毛甾醇原料的含量测定。
     

关键词: font-size:medium, ">羊毛甾醇;高效液相色谱法;含量测定

Abstract: Objective: To establish a method for the determination of lanosterol.Methods: The experiment used an InfinityLabPoroshell 120 Ec-C18(4.6×150 mm, 5 μm )chromatographic column. Methanol was used as the mobile phase and the flow rate was 1.0 mL·min-1. The detection wavelength was 210 nm.The injection volume was 10 μL.The column temperature is 35 ℃.Results: The lanosterol peak can be completely separated from other impurity peaks, and the detection limitation value is 0.008 mg·mL-1. The concentration range 0.048 49-0.113 1 mg·mL-1 with a good linear relationship with the peak area (R2:1.000 0)and the RSD is 1.2%.Meanwhile,the columnswitched to GL Sciences Intertsil ODS-SP C18(4.6×150 mm,5 μm) chromatographic column to determine the RSD of the reference substance peak area is 0.30%. Conclusion: The established method is fast, simple, sensitive and specific, and can be used for the determination of lanosterol quality.
   

Key words: Lanosterol, High performance liquid chromatography(HPLC), Content determination

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