关键词: font-size:medium, ">中药饮片；金银花；微生物污染；控制菌检查；菌种鉴定；16S rRNA基因

Abstract: Objective：To investigate the microbial contamination of 34 batches of Lonicera Flos through the microbial limit test. Methods：According to ChP2020,total aerobic microbial count(TAMC),heat-resistant microbial count (HRMC),total yeasts andmolds count(TYMC) and bile-tolerant G- bacteria count of 34 batches of Lonicera Flos were determined. Escherichia coli and Salmonella.spp were also tested. The bacteria separated from selective mediumwere identified by VITEK2 Compact 30 system. Phylogenetic trees were constructed by V1~V3 region sequences of 16S ribosomal RNA for homology analysis. Results：The average value of lgTAMC was 3.7,the average value of lgHRMC was 1.6 and the average value of lgTYMC was 1.8,the detection rate for bile-tolerant Gram-negative bacteria(N>10)was41.2%.E.coli and Salmonella.spp were not found. However, P.aeruginosa was found in 1 batches of sample. 51 bacterial strains were separated from Control bacteria tests. Pantoea. spp and E. cloacae complex were accounted for about 66.7%（34/51) in all isolated strains. Homology analysis showed that Pantoea.spp could be divided into four groups, and E.cloacae complex could be divided into three groups. Conclusion：The results of microbial limit tests of 34 batches of Lonicera Flos met test requirements, Pantoea.spp and E.cloacae complex were the common and some bacterial were homologous in TCM decoction piecesLonicera Flos. It was necessary to carry out further research on risk assessment, the quality of microbial limit standards and the control measures of TCM should be strengthened.

Key words: TCM decoction piece, Lonicera Flos, Microbialenumeration, Control bacteria tests, Strain identification;16S ribosomal RNA