关键词: font-size:medium, ">去甲乌药碱；怀山药；超高效液相色谱-串联质谱（UPLC-MS-MS

Abstract: Objective：A method for determination of higenamine in Huai rhizoma dioscorea by ultra-performance liquid chromatography tandems mass spectrometry ( UPLC-MS-MS) was established. Methods：Samples were extracted with 0.1% perchloric acid solution、 purified by MCX SPE column and eluted with 5% ammonia methanol solution. After the eluent was blown dry with nitrogen in the water bath， it was redissolved with 50% methaool aqueous solution(containing 0.1% formic acid).Waters BEH C18 ultra high performance chromatographic column (150 mm×2.1 mm，1.7 μm) was used for seperation .The mobile phase was acetonitrile-0.1% formic acid aqueous solution by gradient elution with the column temperature of 30 ℃ and the flow rate of 0.3 mL·min-1.Fenoterol was used as the internal standard for quantification. Results：Higenamine showed a good linearity ( r=0.999 3) in the range of 1~100 μg·L-1.The LOD for higenamine was 0.3 μg·kg-1，The LOQ for higenamine was 1.0 μg·kg-1.The recovery ranged from 86.7%～92.3% and RSD ranged from 2.1% to 4.2%. Conclusion：This method is suitable for determination of higenamine in Huai rhizoma dioscorea.

Key words: Higenamine, Huai rhizoma dioscorea, Ultra-performance liquid chromatography tandems mass spectrometry（UPLC-MS-MS）