关键词: font-size:medium, ">滑石粉烫制貂肾；免疫亲和柱；黄曲霉毒素

Abstract: Objective：In this study， based on immunoaffinity column purification sample， the content of aflatoxin B1， B2， G1， G2 in Processed Mustela was simultaneously determined by photochemical derivatization HPLC-fluorescence detection， and the risk was evaluated. Methods：The sample was extracted with 70% methanol， purified by immunoaffinity column，separated by HPLC， and derivatized by photochemical column. The content of aflatoxin in the sample was determined by fluorescence detector. Risk assessment of harmful residues of aflatoxin in mink kidney was carried out by exposure boundary ratio. Results： The results showed that the linear range of aflatoxin B1 was 0.010 4-0.052 0 ng (r=0.999 9)， the linear range of aflatoxin B2 was 0.003 8-0.019 0 ng (r=0.999 8)， the linear range of aflatoxin G1 was 0.010 8-0.054 0 ng (r=0.999 8)， and the linear range of aflatoxin G2 was 0.003 8-0.019 0 ng (r=0.999 8). The linear relationship was good， the recovery was 89.31%-99.54%， and the RSD was≤3.1%. Conclusion： The established detection method is simple， sensitive， reproducible and accurate. It can be used for the determination of aflatoxin in mink kidney. The risk assessment of aflatoxin in mink kidney has low risk to human health.

Key words: font-size:medium, ">Mustela scalded with talcum powder； Immunoaffinity column； Aflatoxi