关键词: font-size:medium, ">小儿肺热平胶囊；质量评价；牛胆粉；羊胆粉；主成分分析；混伪判别

Abstract: Objective： To establish a quality evaluation method of cattle bile powder in Xiaoer feireping capsules. Methods： The content of conjugated cholic acid in Xiaoer feiping Capsules was determined by HPLC-ELSD. The chromatographic analysis was carried out on a Besil 5 C18-H column (4.6 mm×250 mm， 5 μm) with a column temperature of 35 ℃ and a liquid flow-rate of 1.0 mL·min-1 using 0.2% trifluoroacetic acid solution and acetonitrile as the mobile phase with a linear gradient. An ELSD was used with a nitrogen flow-rate of 3.0 L·min-1，at a drift tube temperature of 110 ℃. The ratio of taurocholic acid to glycolic acid was determined in addition， the principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis(OPLS-DA) of each batch were carried out to obtain the components that affected the consistency between batches. Results： The content of conjugated cholic acid varied considerably between the two manufacturers. PCA combined with OPLS-DA screened out the ratio of taurocholic acid to glycolic acid that had the greatest impact on batch consistency， the ratio in company A was greater than 2.0， and the ratio in company B was less than 2.0. Conclusion： The established method can quickly and effectively evaluate the quality of cattle bile powder in Xiaoer feireping capsules and identify whether there is any false feeding of sheep bile powder.
   

Key words: font-size:medium, ">Xiaoer feireping capsule； Quality evaluation； Cattle bile powder； Sheep bile power； Principal component analysis； Adulteration detection