关键词: font-size:medium, ">活血止痛胶囊；黄曲霉毒素；高效液相色谱；光化学衍生；风险评估

Abstract: Objective： To investigate the feasibility of determination of aflatoxins in Huoxuezhitong capsules by high performance liquid chromatography (HPLC) coupled with post-column photochemical derivatization， and to establish a method for determination of aflatoxin B1， B2， G1 and G2 in Huoxuezhitong capsules. The contamination status was checked to provide a basis for the supervision of aflatoxin pollution of Chinese patent medicine. Methods： The content of aflatoxins in 113 batches of Huoxuezhitong capsules from three manufacturers was determined by HPLC coupled with post-column photochemical derivatization， and the results were analyzed. Results： The recoveries of aflatoxin B1， B2， G1 and G2 in Huoxuezhitong capsules were within 75% -105%. Among 113 batches of samples， aflatoxins were detected in 90 batches， with a detection rate of 79.6%， of which 4 batches exceeded the standard in varying degrees（AFB1>5 μg·kg-1，AFs>10 μg·kg-1）. Conclusion： The HPLC method coupled with post-column photochemical derivatization is accurate and reproducible， which can be used for the determination of aflatoxins in Huoxuezhitong capsules. Aflatoxin pollution in some batches of Huoxuezhitong capsules is serious and there are potential safety hazards， which should be paid attention to by manufacturers to ensure the safety of medication.

Key words: font-size:medium, ">Huoxuezhitong capsules； Aflatoxins； High performance liquid chromatography（HPLC）； Photochemical derivatization； Risk assessmen