关键词: font-size:medium, ">九味羌活丸；指纹图谱；超高效液相色谱；化学模式识别；质量评价

Abstract: Objective： Toestablish UPLC (Ultra-Performance Liquid Chromatography) fingerprint chromatograms for JiuweiQianghuo Pills and conduct chemical pattern recognition.Methods：Waters Acquity UPLC HSS T3 column (2.1 mm × 100 mm， 1.8 μm) was used. Gradient elution was performed with 0.1% phosphoric acid and acetonitrile. The injection volume was 1 μL， the column temperature was set at 40°C， the flow rate was 0.35 mL·min-1， and detection was carried out at 254 nm. Twelve batches of JiuweiQianghuo Pills were analyzed using traditional Chinese medicine fingerprint similarity analysis software and chemical pattern recognition techniques.Results：By comparing the 12 batches of samples， 24 common peaks were identified. UPLC fingerprint chromatograms of JiuweiQianghuo Pills were established， and 13 components were identified. The similarity values among the 12 batches of samples were all above 0.90.The 12 batches of samples were divided into 3 categories by cluster analysis and principal component analysis. The orthogonal partial least square method and discriminant analysis were used to predict that 2 of them were the key factors causing the difference between batches and could be used as the quality signature components of this product.Conclusion：The established UPLC fingerprint chromatograms for JiuweiQianghuo Pills can comprehensively evaluate the intrinsic quality of the product. Combined with chemical pattern recognition analysis， they can provide reference data for setting quality standards for JiuweiQianghuo Pills.

Key words: font-size:medium, ">JiuweiQianghuo Pills；Fingerprint chromatograms；UPLC（Ultra-High Performance Liquid Chromatography）；Chemical pattern recognition；Quality evaluation