关键词: font-size:medium, ">参葛补肾胶囊；总黄酮醇苷；高效液相色谱；一测多评法

Abstract: Objective： To develop a HPLC method for determination of icariin， epimedin A， epimedin B， epimedin C in Shenge Bushen capsule. Methods： The chromatographic was performed on a Waters ACQUITY UPLC HSS T3 C18 column(2.1×100 mm， 1.8 μm)， with acetonitrile(A)-0.05% phosphoric acid solution at the flow rate of 0.3 mL·min-1. Detection wavelength was set at 320 nm， the column temperature was maintained at 30 ℃， and the injection volume was 2 μL. The relative correction factors(fs/k) of the other three components was calculated by slope correction method with icariin as internal reference substance. Results： The linear range of icariin， epimedin A， epimedin B and epimedin were 7.13~713.38 μg·mL-1 (r=0.999 9)， 1.28~128.40 μg·mL-1 (r=0.999 9)， 5.11~510.80 μg·mL-1 (r=0.999 9)， 4.36~435.67 μg·mL(r=0.999 9)， respectively. The average recoveries were 104.56%， 99.69%， 100.00%， 98.59%， and the RSDs were 1.4%， 2.7%， 1.2%， 2.8%， respectively. By slope correction method， fs/k of epimedin A， epimedin B and epimedin C were 1.31， 1.19， 1.17. There were no significance difference in content determination results between two correction methods of QAMS and external standard(P>0.05). Conclusion： The method is simple， accurate and sensitive， and can be used for the quality control study of total Flavonol Glycosides in Shenge Bushen Capsule.

Key words: font-size:medium, ">Shenge Bushen capsules；Total flavonol glycosides； High performance liquid chromatography(HPLC)；Quantitative analysis of multi-components by single marker(QAMS)