关键词: font-size:medium, ">熟大黄配方颗粒；土大黄苷；番泻苷A

Abstract: Objective： To found a method for the identification of rhaponticin and sennoside A in Stewed Rhubarb Granule by LC-MS.Methods： An HPLC-MS /MS in MRM mode was adopted. Welch UHPLC XB-C18(2.1 mm×150 mm，1.8 μm) column was applied with methanol-0.1% phosphoric acid solution as the mobile phase.The Liquid phase flow velocity was 0.3 mL·min-1，The temperature of colum was 35 ℃. The MS/MS system was operated by using an electrospray ionization probe. Scan mode was in multiple reaction ion monitoring(MRM) mode. Results： Limits of detection were 2.0 ng·mL-1 for Rhaponticin and 8.0 ng·mL-1 for Sennoside A.Rhaponticin and Sennoside A weren′t found in 8 batches of samples. Conclusion： The method is exclusive and could not only identify the true or false Stewed Rhubarb，but also examine the purity. It′s necessary for the quality control of Stewed Rhubarb Granule.

Key words: font-size:medium, ">Stewed Rhubarb Dispensing Granule；Rhaponticin； Sennoside A