关键词: font-size:medium, ">多重耐药鲍曼不动杆菌(MDR-Ab)； 生物膜； 亚抑菌浓度； 美罗培南

Abstract: Objective： To investigate the effect of sub-inhibitory concentrations of meropenem on the biofilm action of multi-drug resistant Acinetobacter baumannii(MDR-Ab). Methods： The lowest inhibitory concentration(MIC) of meropenem was measured by broth microdilution. Group A: The biofilm model was constructed with 96-well plate， The mature biofilm was destroyed with meropenem of 1/4 MIC， 1/2 MIC and MIC in experimental groups a， b and c， respectively， Group d control group cultured without meropenem for 48 h， 96 h， Analysis of bacterial biofilms by crystal violet staining， The OD value of the bacterial biofilm at the wavelength of 560 nm was then measured by a multi-function microplate reader. Group B: The bacterial solution was incubated together with meropenem， Meropenem was added 1/4 MIC， 1/2 MIC， MIC to the sub-inhibitory concentration in experimental groups X， Y and Z and cultured MDR-Ab， Group W control group was cultured without meropenem for 48h， 96h， Biofilms were determined by the same method. Results： The OD values of the biofilm in group a， b and c in group A and the OD values in the control group d and the OD values in significant difference(P<0.05)， but no significant difference in 96 h(P>0.05). The OD values in group X， Y， Z and the OD value of control W in 48 h and 9 6 h in group B(P<0.05). Conclusion： MDR-Ab has biofilm formation. Group A: meropenem is able to destroy mature biofilm and increases with concentration. Group B: MDR-Ab biofilm formation at MIC and 1/2 MIC， may induce biofilm formation at 1/4 MIC.

Key words: Multiple drug-resistant Acinetobacter baumannii, biofilm, subinhibitory concentration, meropenem