关键词: font-size:medium, ">肝素钠；亚甲基蓝；紫外分光光度法

Abstract:  Objective： Evaluation of the content and activity of heparin sodium with different potencies. Methods： An ultraviolet spectrophotometric method for the determination of heparin sodium concentration was developed and the effect of different potencies of heparin sodium on the determination was investigated. Results： At the wavelength of 664 nm， the absorbance measured after its reaction with an equal amount of methylene blue showed a good linear relationship with the concentration of sodium heparin when the concentration of heparin sodium was in the range of 0-2.00 mg·L-1. The correlation coefficients of the linear equations for 150 USP units·mg-1， 185 USP units·mg-1， and 205 USP units·mg-1 heparin sodium were 0.999 6， 0.999 2， and 0.999 0， respectively. In addition， it was found that there was a positive correlation between the potency of heparin sodium and the slope of the linear equation， that is， as the potency of heparin sodium increased， the slope also increased. Conclusion： The method developed in this study allows for simple， rapid， and easily controlled quantitative determination of heparin and indirect investigation of heparin activity in large samples.

Key words: font-size:medium, ">Heparin Sodium； Methylene Blue； Ultraviolet Spectroscopy