关键词: font-size:medium, ">活血止痛膏；体外经皮渗透；丁香酚； 丹皮酚；水杨酸甲酯

Abstract: Objective：To establish a method for the determination of eugenol， paeonol and methyl salicylate in transdermal receiving fluid， and to determine and study the in vitro transdermal permeable components of Huoxue Zhitong ointment. Methods： Franz diffusion cell method was used to investigate the transdermal components and regularity of Huoxue Zhitong ointment in vitro. The transdermal components were determined by UPLC and gas chromatography， and the transdermal transmittance of eugenol， paeonol and methyl salicylate in the transdermal receiving fluid was determined. Four different transdermal receiving solutions were compared according to the cumulative transmittance of the components in 24 hours， the parallelism of the measured components and the microstructure of the skin tissue. Results & Conclusion： The transdermal components of Huoxue Zhitong ointment are paeonol， eugenol and methyl salicylate. The cumulative permeability of eugenol， paeonol and methyl salicylate is higher than that of ethanol 0.9% NaCl solution 3∶7， polyethylene glycol 0.9% NaCl solution 2∶8 and as the receiving solution. The parallelism of the percutaneous permeability of effective components and the microstructure of experimental skin tissue are better than that of ethanol propylene glycol 0.9% NaCl solution(2∶2∶6). Therefore， ethanol polyethylene glycol 0.9% NaCl solution(2∶2∶6) is selected as the receiving solution.

Key words: font-size:medium, ">Huoxuezhitong ointment； In vitro percutaneous penetration； Eugenol； Paeonol； Methyl salicylate