关键词: font-size:medium, ">大黄通便片；土大黄苷；薄层色谱（TLC）；高效液相色谱（HPLC）；超高效液相色谱-质谱联用（UHPLC-MS/MS）

Abstract: Objective： To establish a method for the qualitative screening and quantitative determination of rhein in Dahuang Tongbian tablets. Methods： TLC was used for preliminary screening， HPLC was used for quantitative determination， and UHPLC-MS was used for qualitative confirmation. Results： TLC test results showed strong specificity and good durability. HPLC results showed that the mass concentration of rhein showed a good linear relationship in the range of 2.59~259.6 μg·mL-1 (R2=0.999 9). RSDS of precision， repeatability and stability tests were all less than 2.0%. The average recovery was 97.89% (RSD=0.61%). The confirmed results of UHPLC-MS/MS were consistent with TLC and HPLC， and the extracted ion pairs with the same retention time of the positive samples and the control products of rhein (421.1→258.9， 421.1→199.0). Conclusion： The method is sensitive and accurate， and can be used as a method for the detection of illegal components of rhein in Dahuang Tongbian tablets.

Key words: font-size:medium, ">Dahuang Tongbian Tablets；Rhaponticin； Thin Layer Chromatography（TLC）； High Performance Liquid Chromatography(HPLC)； Ultra-High-Performance Liquid Chromatography Mass Spectrometer(UHPLC-MS/MS)