Abstract: Objective: To establish a dissolution method of Azithromycin for Suspension. To investigate the in vitro dissolution of Azithromycin for Suspension from ten manufacturers to provide information for drug quality control and clinical medication. Methods: 500 ml phosphate buffer (pH6.0) was adopted as dissolution medium with paddle method setting at 50 r?min-1. HPLC method was used to test the cumulative dissolution at different times and draw dissolution profiles. Results: The dissolution profiles of Azithromycin for Suspension from ten manufacturers were significantly different, which more really reflect the dissolution processes. Conclusion: The method is simple and accurate which can be used in the determination of dissolution of Azithromycin for Suspension. There was a significant difference in dissolution rate of different manufacturers because of preparation prescription and production engineering.