关键词: font-size:medium, ">大黄碳酸氢钠片；复方龙胆碳酸氢钠片；土大黄苷；超高效液相色谱-质谱联用法

Abstract: Objective： To establish a method for quantitative determination of indicative composition rhaponiticin from counterfeit Rheum palmatum in rhubarb sodium bicarbonate tablets， compound gentian and sodium bicarbonate tablets. Methods： 7 batches of Rhubarb Sodium Bicarbonate Tablets， 4 batches of compound gentian and sodium bicarbonate tablets were collected， totaling 11 batches. The content of rhaponiticin was determined by UPLC-MS/MS method. Results： UPLC-MS/MS methodology investigation showed that the linear range of rhaponiticin was 0.5-500 ng·mL-1（r=0.999 2）.The detection limit and quantitative limit were 5，15 ng·g-1. RSDs of precision，reproducibility and stability tests were all lower than 1％.Average recovery of rhubarb sodium bicarbonate tablets was 95.16％（RSD=1.07％， n=6）， average recovery of compound gentian and sodium bicarbonate tablets was 96.13％（RSD=1.46％，n=6）. The contents of rhaponiticin in 4 batches of samples were 7.6～3 392.1 μg·g-1. Conclusion： UPLC-MS/MS for content determination is simple，sensitive and reliable，and can be used for quantitative determination of rhaponiticin in Rhubarb sodium bicarbonate tablets， compound gentian and sodium bicarbonate tablets. 

Key words: font-size:medium, ">Rhubarb sodium bicarbonate tablets； Compound gentian and sodium bicarbonate tablets； Rhaponiticin； UPLC-MS/MS