Abstract: [Abstract]Objective： To study the application of rocuronium in older patients with laparoscopic cholecystectomy (LC). Methods： 89 cases who intend to perform LC surgical treatment of liver and gallbladder surgery were randomly divided into two groups. 45 cases in the study group were given rocuronium injection， and the other 44 cases in the control group were given cisatracurium besilate injection. Results：1， 3， 5， 10 min histamine concentrations、 HR value after administration in study group was significantly lower than in control group (P<0.05)， 1， 3， 5， 10 min MAP after administration in study group was significantly higher than in control group (P<0.05). The muscle relaxants onset time (126.3±9.3) s of patients in study group is significantly shorter than in control group (194.5±11.0) s (P<0.05). The duration (49.2±6.9) min in study group were significantly longer than in control group (32.8±6.3) min (P<0.05). The incidence of adverse events in study group （8.89%） was significantly lower than in control group（31.82%）(P<0.05). Conclusion： Rocuronium injection in elderly patients undergoing LC does not increase histamine release. It will reduce adverse reactions in patients with surgery， and provide with rapid onset， long duration， hemodynamic stability.