• 中国核心期刊(遴选)数据库收录期刊
  • 中文科技期刊数据库收录期刊
  • 中国期刊全文数据库收录期刊
  • 中国学术期刊综合评价数据库统计源期刊等

• 评价技术与方法 • 上一篇    下一篇

问荆药材中异斛皮苷HPLC法测定的不确定度分析

赵艳,崔业波,马晓静,韩凤   

  1. 四平市食品药品检验所,四平市食品药品检验所,四平市食品药品检验所,四平市食品药品检验所
  • 收稿日期:2017-08-08 修回日期:2017-08-08 出版日期:2017-12-25 发布日期:2017-12-25

Uncertainty Analysis of Quercetin Determination of Isoquercitrin in Equisetum arvens L. by HPLC

  1. Siping City Institute for Food and Drug Control,Jilin Siping 136000,,,
  • Received:2017-08-08 Revised:2017-08-08 Online:2017-12-25 Published:2017-12-25

摘要: 目的:探讨问荆药材中异斛皮苷含量的不确定度及影响因素。方法:通过分析测量过程中影响测量结果的因素,采用HPLC法测定中的方法验证数据及代表样品的实验数据,经统计学方法,量化不确定度分量,合成不确定度和扩展不确定度。结果与结论:异斛皮苷的扩展不确定度为0.04 mg?g-1,测量不确定度可用于问荆药材标准的制定;不确定的评定方法确立对中药质量标准的研究具有重要意义。

Abstract: Objective: To analyze the uncertainty of isoquercitrin in Equisetum arvens L.(E. arvens L.) by HPLC. Methods: The source of uncertainty was confirmed by analyzing the HPLC determination process. The uncertainty components were quantified by using the validation data of assay and experimental data of sample representation studies and the extended uncertainty and confidence level were finally obtained. Results and Conclusion: The extended uncertainty of isoquercitrin was 0.04 mg?g-1. The uncertainty analysis method is suitable for the standard limit formulation for E. arvens L. and it is important to establish uncertainty analysis methods for tradition Chinese medicines.