Abstract: Objecetive：To establish methods for determination of heavy metals including plumbum， cadmium， arsenic， mercury and cuprum in Pollen Brassicae Cammestris. Methods：Determinatin of plumbum， cadmium， and cuprum was conductedby atomic absorption spectrometry， Determination of arsenicwas conducted by hydride generation system. Determination of mercury was conducted by Cold vapor absorption. And the methods were validated. Results：The results were reasonable and effective through the detection and methodological study of Pb， Cd， As， Hg and Cu. The linear correlation coefficients were greater than 0.995. The average recoveries were 91%， 82%， 86%， 109%， 80%. Conclusion：The methods in the research are simple， accuracy， reproducible and can be used for the determination of the five heavy metals in Pollen Brassicae Cammestris， and provide more comprehensive quality control methods for Pollen Brassicae Cammestris.