Abstract: Objective： To establish a thin layer chromatography identification method for hazelnut flowers and to establish a HPLC method for the determination of quercetin and kaempferol in Corylus heterophylla Fisch. Methods： The quercetin in Corylus heterophylla Fisch was qualitatively identified on silica gel G plate of thin-layer chromatography. Chromatographic conditions were used： Inertsil ODS-SP C18 (4.6 mm×250 mm， 5 μm)； mobile phase was methanol -0.4% phosphoric acid (48∶52) The detection wavelength was 360 nm； the column temperature was 30 ℃； and the flow rate was 1.0 mL·min-1. Results： The TLC identification method has good reproducibility and strong specificity. The linearity of quercetin and kaempferol in the content determination was good (r>0.999 5). The recoveries were 98.31% (RSD 1.3%) and 96.80% (RSD 0.96%)， respectively. Conclusion： The establishment method is simple， prepared and reproducible， and can provide a reference for the application of the medicine.