关键词: font-size:medium, ">痢泻灵片；苦参；苦参碱；氧化苦参碱；含量测定；高效液相色谱

Abstract: Objective：Toestablish an HPLC method for the determination of matrin andoxymatrin in Li xieling tablet，analyze and evaluate the market products. Methods：High performance liquid chromatography was used to examine the contents of matrine and oxymatrine in Lixieling tablets，Using symmetry C18 column（5 μm，4.6 mm×250 mm），the mobile phase was acetonitrile(A)-0.2% phosphate buffer (B) with gradient elution at a flow rate of 0.8 mL·min-1， The temperature for chromatographic column was 30 ℃ and the detection wavelength was 220 nm.Results：Baseline separation can be achieved for matrin，oxymatrin in Li xie ling tablet. The method had a good linearity in the ranges of 0.395 6～6.328 8(r=1.000 0)，0.355 8～5.692 2 μg(r=1.000 0)，The average recoveris(n=6) were99.32%、98.03%.Conclusion：Thisassay isproved to be reproducible，spefic and practical.It is suitable for the determination of matrine and oxymatrine in Lixieling tablets， and can effectively control the product quality.Between 0.32 and 1.50mg， it showed that the quality of the products among the different manufacturing enterprises was greatly different.

Key words: Li xie ling tablet, Radix sophorae flavescentis, Matrin, Oxymatrin;Content determination, HPLC