关键词: font-size:medium, ">和中降浊调控丸；橙皮苷；苍术素；含量测定

Abstract: Objective： To develop a high-performance liquid chromatography(HPLC) analytical approach enabling the simultaneous determination of the contents of two active ingredients in Hezhong Jiangzhuo control pills， thus providing a reference for its quality control. Methods：An ACES C18 chromatographic column(250 mm×4.6 mm， 5 μm) was employed. The mobile phase consisted of acetonitrile and water for gradient elution. The flow rate was set at 1.0 mL·min-1， and the column temperature was maintained at 35 ℃. Dual-wavelength detection was performed at 283 nm and 340 nm， with an injection volume of 5 μL. Results： For atractylodin and hesperidin in Hezhong Jiangzhuo control pills， excellent linear relationships between their contents and peak areas were observed within the ranges of 0.0214 μg to 0.2672 μg and 0.0768 μg to 0.9597 μg respectively(r2 ≥0.999 9). The relative standard deviation(RSD) values obtained from the precision， stability， and repeatability tests were all less than 2.0%. The average recoveries of atractylodin and hesperidin were 99.64% and 101.7% respectively(n=6). In samples from three different packaging batches， the measured contents of atractylodin and hesperidin were 0.21 mg·g-1 and 1.27 mg·g-1， 0.22 mg·g-1 and 1.19 mg·g-1， as well as 0.22 mg·g-1 and 1.23 mg·g-1 respectively. Conclusion：This method is relatively straightforward to operate and exhibits high sensitivity. The results are stable and reliable， making it suitable for the quality control of atractylodin and hesperidin in Hezhong Jiangzhuo control pills.
 

Key words: font-size:medium, ">Hezhong jiangzhuo control pills； Hesperidin； Atractylotin； Content determination